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Anti-ING5 Antibody (6I821)
😃Good
Catalog No. TMAY-02670
Alias p28ING5, inhibitor of growth family, member 5
Anti-ING5 Antibody (6I821) is a Rabbit antibody targeting ING5. Anti-ING5 Antibody (6I821) can be used in WB,ELISA,IP.
Anti-ING5 Antibody (6I821)
Anti-ING5 Antibody (6I821)
😃Good
Catalog No. TMAY-02670Alias p28ING5, inhibitor of growth family, member 5
Anti-ING5 Antibody (6I821) is a Rabbit antibody targeting ING5. Anti-ING5 Antibody (6I821) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-ING5 Antibody (6I821) is a Rabbit antibody targeting ING5. Anti-ING5 Antibody (6I821) can be used in WB,ELISA,IP. |
| Alias | p28ING5, inhibitor of growth family, member 5 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6I821 |
| Reactivity | Human |
| Verified Activity | 1. ING5 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-ING5 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ING5 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 28 kDa. -Observed band size: 32 kDa. 2. Anti-ING5 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:28 kDa. -Observed band size:34 kDa(We are unsure as to the identity of these extra bands.) |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ING5 (rh ING5; TMPY-02981; Q8WYH8-2; Glu36-Gln84). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ING5 belongs to the ING family. It contains 1 PHD-type zinc finger and is a component of the HBO1 complex. HBO1 complex has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of PHF15, PHF16, and PHF17. ING5 also is a component of the MOZ/MORF complex which is composed at least of ING5, KAT6A, KAT6B, MEAF6, and one of BRPF1, BRD1/BRPF2, and BRPF3. It interacts with EP300 and TP53. MOZ/MORF complex has a histone H3 acetyltransferase activity. Through chromatin acetylation, ING5 may regulate DNA replication and may function as a transcriptional coactivator. It interacts with H3K4me3 and to a lesser extent with H3K4me2. ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. It can bind TP EP300/p300, a component of the histone acetyltransferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human ING5 Protein (TMPY-02981) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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