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Anti-CD16/FCGR3 Antibody (6W413)

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Catalog No. TMAY-03235
Alias Fc receptor, IgG, low affinity III, CD16

Anti-CD16/FCGR3 Antibody (6W413) is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (6W413) can be used in WB,ELISA,IP.

Anti-CD16/FCGR3 Antibody (6W413)

Anti-CD16/FCGR3 Antibody (6W413)

😃Good
Catalog No. TMAY-03235Alias Fc receptor, IgG, low affinity III, CD16
Anti-CD16/FCGR3 Antibody (6W413) is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (6W413) can be used in WB,ELISA,IP.
Pack SizePriceAvailabilityQuantity
20 μL$92 7-10 days
100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD16/FCGR3 Antibody (6W413) is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (6W413) can be used in WB,ELISA,IP.
AliasFc receptor, IgG, low affinity III, CD16
Ig Type
Monoclonal Rabbit IgG
Clone
6W413
Reactivity
Mouse
Verified Activity
1. Anti-FCGR3 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: C6 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:29 kDa.

-Observed band size:38 kDa(We are unsure as to the identity of these extra bands.)

2. mFCGR3 was immunoprecipitated using:

-Lane A:0.5 mg RAW246.7 Whole Cell Lysate.

-4µL anti-mFCGR3 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

-Primary antibody:

-Anti-mFCGR3 rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size: 29 kDa.

-Observed band size:50 kDa
Application
WB,ELISA,IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16 / FCGR3 (rM CD16 / FCGR3; TMPY-01834; P08508-1; Met 1-Thr 215).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundFc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Mouse CD16 / FCGR3 protein (TMPY-01834)
Antigen Species
Mouse
Biology Area
Fc Receptors
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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