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Anti-CD16/FCGR3 Antibody (6W413)
😃Good
Catalog No. TMAY-03235
Alias Fc receptor, IgG, low affinity III, CD16
Anti-CD16/FCGR3 Antibody (6W413) is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (6W413) can be used in WB,ELISA,IP.
Anti-CD16/FCGR3 Antibody (6W413)
Anti-CD16/FCGR3 Antibody (6W413)
😃Good
Catalog No. TMAY-03235Alias Fc receptor, IgG, low affinity III, CD16
Anti-CD16/FCGR3 Antibody (6W413) is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (6W413) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Mouse |
| Alias | Fc receptor, IgG, low affinity III, CD16 |
| Ig Type | Rabbit IgG |
| Clone | 6W413 |
| Reactivity | Mouse |
| Verified Activity | 1. Anti-FCGR3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: C6 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:29 kDa. -Observed band size:38 kDa(We are unsure as to the identity of these extra bands.) 2. mFCGR3 was immunoprecipitated using: -Lane A:0.5 mg RAW246.7 Whole Cell Lysate. -4µL anti-mFCGR3 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-mFCGR3 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 29 kDa. -Observed band size:50 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16 / FCGR3 (rM CD16 / FCGR3; TMPY-01834; P08508-1; Met 1-Thr 215). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Mouse CD16 / FCGR3 protein (TMPY-01834) |
| Antigen Species | Mouse |
| Biology Area | Fc Receptors |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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