Anti-MAG Polyclonal Antibody 2 is a Rabbit antibody targeting MAG. Anti-MAG Polyclonal Antibody 2 can be used in WB,ELISA,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Cerebellum tissue lysates
Lane 3: Rat Spinal cord tissue lysates
Primary: Anti-MAG (TMAB-08508) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 98 kDa
2. 25 μg total protein per lane of various lysates (see on figure) probed with MAG polyclonal antibody, unconjugated (TMAB-08508) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (TMAB-08508) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (TMAB-08508) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (TMAB-08508) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (TMAB-08508) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (TMAB-08508) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (TMAB-08508) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; ELISA: 1:5000-10000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100

Polyclonal

KLH conjugated synthetic peptide: human MAG

Human

MAG

Myelin-associated glycoprotein

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.