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Anti-USP6 Antibody (1Q774)
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Catalog No. TMAH-01240
Anti-USP6 Antibody (1Q774) is a Mouse antibody targeting USP6. Anti-USP6 Antibody (1Q774) can be used in ELISA, WB, IF.
Anti-USP6 Antibody (1Q774)
Anti-USP6 Antibody (1Q774)
😃Good
Catalog No. TMAH-01240
Anti-USP6 Antibody (1Q774) is a Mouse antibody targeting USP6. Anti-USP6 Antibody (1Q774) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Ig Type | IgG1 |
| Clone | 1Q774 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: USP6 antibody at 1:1000 -Lane 1: MCF-7 whole cell lysate -Lane 2: THP-1 whole cell lysate -Lane 3: SH-SY5Y whole cell lysate -Lane 4: Jurkat whole cell lysate -Lane 5: HEK293 whole cell lysate -Lane 6: Hela whole cell lysate -Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution -Predicted band size: 89, 122, 159 KDa -Observed band size: 89, 122, 159 KDa -Exposure time: 1min 2. Immunofluorescence staining of HeLa cells with TMAH-01240 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). |
| Application | ELISA, WB, IF |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Research Background | Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Ubiquitin carboxyl-terminal hydrolase 6 Protein (348-535AA) |
| Antigen Species | Human |
| Gene ID | 9098 |
| Uniprot ID | |
| Biology Area | Cell Biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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