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Synonyms: Peptide Ready, MHC, HLA-A*33:03


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $96 | 7-10 days | 7-10 days | |
| 10 µg | $147 | 7-10 days | 7-10 days | |
| 20 µg | $228 | 7-10 days | 7-10 days | |
| 50 µg | $419 | 7-10 days | 7-10 days | |
| 100 µg | $665 | 7-10 days | 7-10 days | |
| 200 µg | $1,050 | 7-10 days | 7-10 days | |
| 500 µg | $1,960 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). |
| Description | Peptide Ready HLA-A*33:03&B2M Monomer Protein, Human, MHC (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is VCU43110.1(HLA-A*33:03)&P61769(B2M). |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | VCU43110.1(HLA-A*33:03)&P61769(B2M) |
| Construction | Gly25-Thr305 (HLA-A*33:03) and Ile21-Met119 (B2M) |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, 100 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | Peptide Ready, MHC, HLA-A*33:03 |
| Research Background | HLA-A*33:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*33:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
| Molecular Weight | 48.3 kDa (Predicted); 50-65 kDa (Reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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