Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877)
🥰Excellent
Catalog No. TMAY-02954
Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) is a Mouse antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) can be used in WB,ELISA,ELISA(Cap),IHC-P,FCM.
Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877)
Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877)
🥰Excellent
Catalog No. TMAY-02954
Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) is a Mouse antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) can be used in WB,ELISA,ELISA(Cap),IHC-P,FCM.
Product information is being updated, if you want to purchase, please click the bulk custom button.
Bulk & Custom
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) is a Mouse antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) can be used in WB,ELISA,ELISA(Cap),IHC-P,FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 5T877 |
| Verified Activity | 1. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid mouse monoclonalantibody at 1:1000 dilution. -Lane A:SARS-CoV NucleocapsidProtein(30ng) -Lane B: SARS-CoV NucleocapsidProtein(10ng) -Lane C: SARS-CoV-2 (2019-nCoV) Nucleocapsid-His recombinant Protein(10ng) -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. 2. Immunochemical analysis of Nucleocapsid overexpressed Hek293 Cells were stained with purified anti-SARS-CoV-2 Nucleocapsid Mouse Mab,then a HRP-conjugated second step antibody. (Validation Experiment) 3. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid mouse monoclonal antibody at 1:5000 dilution. -Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B: HEK293 Whole Cell lysate. -Lysates/proteins at 10 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) 4. Flow cytometric analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid Mouse MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. (Validation Experiment) |
| Application | WB,ELISA,ELISA(Cap),IHC-P,FCM |
| Recommended Dose | WB: 1:1000-1:10000; ELISA: 1:1000-1:2000; IHC-P: 1:1000-1:5000; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 (2019-nCoV) Nucleocapsid (TMPY-05664; YP_009724397.2(335Gly/Ala); Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (TMPY-05664) |
| Antigen Species | SARS-CoV-2 |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) | purchase Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) | Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877) cost | order Anti-SARS-CoV-2 Nucleocapsid Antibody (5T877)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.