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Anti-HMGCL Antibody (2T378) is an antibody targeting HMGCL. Anti-HMGCL Antibody (2T378) can be used in ELISA, WB, IHC, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, FCM Reactivity: Human |
Alias | mitochondrial, Hydroxymethylglutaryl-CoA lyase, HMG-CoA lyase, HL, EC 4.1.3.4, 3-hydroxy-3-methylglutarate-CoA lyase |
Ig Type | Rabbit IgG |
Clone | 2T378 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate -All lanes: HMGCL antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 35, 27, 21 kDa -Observed band size: 35 kDa 2. IHC image of TMAH-00560 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. Overlay Peak curve showing Hela cells stained with TMAH-00560 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, WB, IHC, FCM |
Recommended Dose | WB:1:500-1:2000; IHC:1:50-1:200; FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Mitochondrion matrix. Peroxisome. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human HMGCL |
Antigen Species | Human |
Gene ID | 3155 |
Uniprot ID | |
Biology Area | Cancer, Metabolism, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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