B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 814.00 | |
500 μg | 5 days | $ 3,250.00 | |
1 mg | 5 days | $ 5,440.00 |
Description | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Accession Number | P42081-1 |
Synonyms | LAB72, B7-2, B7-2 antigen, FUN-1, CD86 molecule, MGC34413, B70, B72, CD86, BU63, CD28LG2 |
Construction | Recombinant Biotinylated Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu26-Pro247.[Accession |P42081-1] |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
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Please read the User Guide of Recombinant Proteins for more specific information.
B7-2 Protein, Human, Recombinant (His & Avi), Biotinylated BU 63 CD-86 LAB72 B7-2 MGC 34413 B7-2 antigen LAB-72 B-70 FUN-1 MGC-34413 CD86 molecule LAB 72 B-72 B 70 MGC34413 CD 86 B70 B72 FUN 1 CD86 BU63 BU-63 FUN1 CD28LG2 B 72 recombinant recombinant-proteins proteins protein