B7-H3 Protein, Human, Recombinant (His)

Name: B7-H3 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02031
Price: 36 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3) Copy Product Info

Synonyms: CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3

Catalog No. TMPY-02031 Copy Product Info

Purity: 97.6%

B7-H3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48 kDa and the accession number is Q5ZPR3-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$36	-	In Stock
10 µg	$55	7-10 days	7-10 days
20 µg	$86	7-10 days	7-10 days
50 µg	$163	-	In Stock
100 µg	$272	7-10 days	7-10 days
200 µg	$481	7-10 days	7-10 days
500 µg	$1,020	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:97.6%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Immobilized Anti-Human B7H3 antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-H3 / CD276 Protein (ECD,His Tag), the EC50 is 2.5-7.5 ng/mL.
Description	B7-H3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48 kDa and the accession number is Q5ZPR3-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q5ZPR3-1
Construction	A DNA sequence encoding the human CD276 (Q5ZPR3-1) extracellular domain (Met1-Thr461) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 29
Protein Purity	≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3
Research Background	B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

48 kDa (predicted); 68 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.