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B7-H3 Protein, Human, Recombinant (His)

Catalog No. TMPY-02031
Synonyms: CD276 molecule, B7-H3, 4Ig-B7-H3, B7RP-2, B7H3

B7-H3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48 kDa and the accession number is Q5ZPR3-1.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
B7-H3 Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
100 μg In stock $ 272.00
200 μg 5 days $ 481.00
500 μg 5 days $ 1,020.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Immobilized Anti-Human B7H3 antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-H3 / CD276 Protein (ECD,His Tag), the EC50 is 2.5-7.5 ng/mL.

Description B7-H3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48 kDa and the accession number is Q5ZPR3-1.
Species Human
Expression System HEK293 Cells
Tag C-His
Accession Number Q5ZPR3-1
Synonyms CD276 molecule, B7-H3, 4Ig-B7-H3, B7RP-2, B7H3
Construction The Human CD276 (Q5ZPR3-1) extracellular domain (Met1-Thr461) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity ≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.

Molecular Weight 48 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.


In general, Lyophilized powders are shipping with blue ice.

Research Background B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

References and Literature


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B7-H3 Protein, Human, Recombinant (His) CD276 molecule B7-H3 4Ig-B7-H3 B7RP-2 B7H3 recombinant recombinant-proteins proteins protein