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Anti-HDAC9 Antibody (7A288) is an antibody targeting HDAC9. Anti-HDAC9 Antibody (7A288) can be used in ELISA, WB, IHC, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Anti-HDAC9 Antibody (7A288) is an antibody targeting HDAC9. Anti-HDAC9 Antibody (7A288) can be used in ELISA, WB, IHC, IF. |
Ig Type | Rabbit IgG |
Clone | 7A288 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate -All lanes: HDAC9 antibody at 1.54μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 112, 102, 66, 98, 118, 113, 61, 63, 58 KDa -Observed band size: 160 KDa 2. IHC image of TMAH-00538 diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. IHC image of TMAH-00538 diluted at 1:154 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 4. Immunofluorescence staining of Hela cells with TMAH-00538 at 1:51, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, WB, IHC, IF |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human HDAC9 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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