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MSLN/Mesothelin Protein, Cynomolgus, Recombinant (His)

(Synonyms: SMR, MSLN, MPFSMRP, MPF, Mesothelin, CAK1 antigen, CAK1) Copy Product Info

Synonyms: SMR, MSLN, MPFSMRP, MPF, Mesothelin, CAK1 antigen, CAK1

Catalog No. TMPK-01377 Copy Product Info
MSLN/Mesothelin Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33 kDa and the accession number is A0A2K5TW94-1.

Batch Information

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Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Cynomolgus MSLN, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.
Description
MSLN/Mesothelin Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33 kDa and the accession number is A0A2K5TW94-1.
Species
Cynomolgus
Expression System
HEK293 Cells
TagC-His
Accession NumberXP_005590873.4
ConstructionAsp296-Gly580
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsSMR, MSLN, MPFSMRP, MPF, Mesothelin, CAK1 antigen, CAK1
Research Background
Chemical Properties
Molecular Weight33 kDa (predicted). Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: MSLN/Mesothelin Protein, Cynomolgus, Recombinant (His) chemical structure | MSLN/Mesothelin Protein, Cynomolgus, Recombinant (His) molecular weight