IL-27 Protein, Human, Recombinant (His)

IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells, and dendritic cells. IL-27 protein is also referred to as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), and IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives the rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.

Pack Size	Availability	Price/USD
5 μg	In stock	$ 57.00
10 μg	5 days	$ 91.00
20 μg	5 days	$ 147.00
50 μg	5 days	$ 277.00
100 μg	5 days	$ 447.00
200 μg	5 days	$ 791.00
500 μg	5 days	$ 2,600.00

Pack Size

Availability

Price/USD

Quantity

5 μg

In stock

$ 57.00

10 μg

5 days

$ 91.00

20 μg

5 days

$ 147.00

50 μg

5 days

$ 277.00

100 μg

5 days

$ 447.00

200 μg

5 days

$ 791.00

500 μg

5 days

$ 2,600.00

Biological Information

1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc , The EC50 of human IL27RA-Fc is 0.2-0.46 μg/ml. 2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically 23-92 ng/mL.

Description

Species	Human
Expression System	CHO
Tag	His
Accession Number	NP_005746.2&NP_663634.2
Synonyms	IL27p28, IL-27, IL27A, interleukin 27, p28, IL-27A, IL30
Construction	A DNA sequence encoding the IL27 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the N-terminally truncated IL27p28 (IL27A) subunit (NP_663634.2) (Phe 29-Pro 243) linked by an polypeptide linker was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 56 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Lyophilized from sterile 20mM MOPS,150mM NaCl, 0. 05% CHAPS, PH7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells, and dendritic cells. IL-27 protein is also referred to as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), and IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives the rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.