SARS-COV-2 Spike S1 Protein (hFc & Avi)

Name: SARS-COV-2 Spike S1 Protein (hFc & Avi)
Brand: TargetMol
SKU: TMPK-00432
Price: 56 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1) Copy Product Info

Synonyms: Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1

Catalog No. TMPK-00432 Copy Product Info

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 102.6 kDa and the accession number is A0A6G7K2L4.

Pack Size	Price	USA Stock	Global Stock
5 µg	$56	-	In Stock
10 µg	$88	-	In Stock
20 µg	$143	-	In Stock
50 µg	$278	-	In Stock
100 µg	$465	-	In Stock
200 µg	$839	7-10 days	7-10 days
500 µg	$1,850	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Immobilized Human ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA. 2. SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay.
Description	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 102.6 kDa and the accession number is A0A6G7K2L4.
Species	SARS-CoV-2
Expression System	HEK293 Cells
Tag	C-hFc-Avi
Accession Number	A0A6G7K2L4
Construction	Gln14-Arg683
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1
Research Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Chemical Properties

Molecular Weight

102.6 kDa (predicted). Due to glycosylation, the protein migrates to 130-140 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.