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Anti-CD53 Antibody (9H299)
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Catalog No. TMAY-03902
Alias Tspan25, Ox-44, CD53 molecule, AI323659
Anti-CD53 Antibody (9H299) is a Rabbit antibody targeting CD53. Anti-CD53 Antibody (9H299) can be used in WB,ELISA,IP.
Anti-CD53 Antibody (9H299)
Anti-CD53 Antibody (9H299)
😃Good
Catalog No. TMAY-03902Alias Tspan25, Ox-44, CD53 molecule, AI323659
Anti-CD53 Antibody (9H299) is a Rabbit antibody targeting CD53. Anti-CD53 Antibody (9H299) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Cynomolgus |
| Alias | Tspan25, Ox-44, CD53 molecule, AI323659 |
| Ig Type | Rabbit IgG |
| Clone | 9H299 |
| Reactivity | Cynomolgus |
| Verified Activity | 1. Anti-CD53 rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: Raji Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:24 kDa. -Observed band size:35 kDa. 2. cyno CD53 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg Raji Whole Cell Lysate. -2 µL anti-cyno CD53 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-cyno CD53 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size: 34 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus CD53 ( TMPY-03422; G7NW46; Glu107-Asn181). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Cynomolgus CD53 Protein (TMPY-03422) |
| Antigen Species | Cynomolgus |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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