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Anti-CCND1 Antibody (1E128)
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Catalog No. TMAH-00152
Anti-CCND1 Antibody (1E128) is an antibody targeting CCND1. Anti-CCND1 Antibody (1E128) can be used in ELISA, WB.
Anti-CCND1 Antibody (1E128)
Anti-CCND1 Antibody (1E128)
😃Good
Catalog No. TMAH-00152
Anti-CCND1 Antibody (1E128) is an antibody targeting CCND1. Anti-CCND1 Antibody (1E128) can be used in ELISA, WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB Reactivity: Human, Mouse |
| Ig Type | Rabbit IgG |
| Clone | 1E128 |
| Reactivity | Human, Mouse |
| Verified Activity | Western Blot -Positive WB detected in: NIH/3T3 whole cell lysate, SH-SY5Y whole cell lysate, U-87 whole cell lysate -All lanes: Cyclin D1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34 kDa -Observed band size: 34 kDa |
| Application | ELISA, WB |
| Recommended Dose | WB:1:500-1:5000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. Nucleus membrane. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Cyclin D1 |
| Antigen Species | Human |
| Gene ID | 595 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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