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Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)
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Catalog No. TMAY-02970
Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) is a Rabbit antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) can be used in WB,ELISA,ELISA(Det),FCM,ICC/IF,IP.
Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)
Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)
😃Good
Catalog No. TMAY-02970
Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) is a Rabbit antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) can be used in WB,ELISA,ELISA(Det),FCM,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $235 | 7-10 days | |
| 100 μL | $470 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB, ELISA, ELISA(Det), FCM, ICC/IF, IP |
| Ig Type | Rabbit IgG |
| Clone | 2G259 |
| Verified Activity | 1. Flow cytometric analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid Rabbit MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. (Validation Experiment) 2. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid rabbit monoclonal antibody at 1:5000 dilution. -Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B: HEK293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) 3. Immunofluorescence analysis of SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Cells were stained with purified anti-SARS-CoV-2 Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody. (Validation Experiment) 4. SARS-COV-2 Nucleocapsid was immunoprecipitated using: -Lane A:0.5 mg SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B:0.5 mg HEK293 Whole Cell Lysate. -4 µL anti-SARS-COV-2 Nucleocapsid rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -anti-SARS-COV-2 Nucleocapsid rabbit polyclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) |
| Application | WB,ELISA,ELISA(Det),FCM,ICC/IF,IP |
| Recommended Dose | WB: 1:5000-1:20000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; IP: 1-5 µL/mg of lysate; ELISA(Det): 1:1000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (TMPY-05664) |
| Antigen Species | SARS-CoV-2 |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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