CD16a Protein, Human, Recombinant (F176V, His)

Name: CD16a Protein, Human, Recombinant (F176V, His)
Brand: TargetMol
SKU: TMPY-01964
Price: 613 EUR
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: IMD20, IGFR3, FCRIIIA, FCRIII, FCR-10, FCGRIII, FCGR3, FCG3, Fc γ RIIIa, Fc gamma RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16A, CD16) Copy Product Info

Synonyms: IMD20, IGFR3, FCRIIIA, FCRIII, FCR-10, FCGRIII, FCGR3, FCG3, Fc γ RIIIa, Fc gamma RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16A, CD16

Catalog No. TMPY-01964 Copy Product Info

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

CD16a Protein, Human, Recombinant (F176V, His)

Pack Size	Price	EUR Stock	Global Stock
5 µg	43 €	-	In Stock
10 µg	67 €	-	In Stock
20 µg	106 €	-	In Stock
50 µg	206 €	7-10 days	7-10 days
100 µg	347 €	-	In Stock
200 µg	613 €	7-10 days	7-10 days
500 µg	Preferential	7-10 days	7-10 days

For In stock only · Estimated delivery: EUR Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:SDS-PAGE: 96.7%; SEC-HPLC: 99.0%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1. Immobilized Human FcγRIIIA / CD16a (V176) recombinant protein at 2 μg/mL can bind Human IgG1 , the EC50 is 150-450 ng/mL. 2.Loaded Human FcγRIIIA / CD16a (V176) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.83 μM as determined in a BLI assay . 3.Captured Human FcγRIIIA / CD16a (V176) recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 μM as determined in an SPR assay. 4.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (V176) recombinant protein with an affinity constant of 0.78 µM as determined in an SPR assay.
Description	The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells._x000D_ CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P08637-1
Construction	A DNA sequence encoding the extracellular domain (Met1-Gln208) of human CD16a (P08637-1, with natural variant Phe 176 Val) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gly 17
Protein Purity	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	IMD20, IGFR3, FCRIIIA, FCRIII, FCR-10, FCGRIII, FCGR3, FCG3, Fc γ RIIIa, Fc gamma RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16A, CD16
Research Background	The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells._x000D_ CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

Chemical Properties

Molecular Weight

23.3 kDa (predicted); 44.2 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.