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MSLN/Mesothelin Protein, Human, Recombinant (hFc), Biotinylated

(Synonyms: SMRP, MPF, mesothelin) Copy Product Info

Synonyms: SMRP, MPF, mesothelin

Catalog No. TMPY-02875 Copy Product Info
Mesothelin Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 60.7 kDa and the accession number is Q13421-2.

Product Introduction

Bioactivity
Bioactivity
Immobilized Anti-MSLN Antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human MSLN/Mesothelin Protein (Fc Tag), Biotinylated (Cat#TMPY-02875), the EC50 is 0.9-2.7 ng/mL.
Description
Mesothelin Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 60.7 kDa and the accession number is Q13421-2.
Species
Human
Expression System
HEK293 Cells
TagN-hFc
Accession NumberQ13421-2
ConstructionA DNA sequence encoding the human MSLN (Q13421-2) (Glu296-Gly580) was expressed with the fused Fc region of human IgG1 at the N-terminus. The expressed protein was biotinylated in vitro. Predicted N terminal: Glu
Protein Purity
> 85 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsSMRP, MPF, mesothelin
Research Background
Chemical Properties
Molecular Weight60.7 kDa (predicted); 65 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: MSLN/Mesothelin Protein, Human, Recombinant (hFc), Biotinylated chemical structure | MSLN/Mesothelin Protein, Human, Recombinant (hFc), Biotinylated molecular weight