EphB4 Protein, Mouse, Recombinant (His)

Name: EphB4 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01787
Rating: 4.5 (1 reviews)

(Synonyms: Tyro11, Myk1, MDK2, Htk, EPH receptor B4, AI042935) Copy Product Info

Synonyms: Tyro11, Myk1, MDK2, Htk, EPH receptor B4, AI042935

Catalog No. TMPY-01787 Copy Product Info

EphB4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is P54761-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$59	7-10 days	7-10 days
10 µg	$95	7-10 days	7-10 days
20 µg	$155	7-10 days	7-10 days
50 µg	$296	7-10 days	7-10 days
100 µg	$497	7-10 days	7-10 days
200 µg	$987	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized Mouse Ephrin B2hFc at 2 μg/ml (100 μl/well) can bind Mouse EphB4 His, the EC50 of Mouse EphB4 His is 3.5-20 ng/mL.
Description	EphB4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is P54761-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P54761-1
Construction	A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 16
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	Tyro11, Myk1, MDK2, Htk, EPH receptor B4, AI042935
Research Background	Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Chemical Properties

Molecular Weight

59 kDa (predicted); 70 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.