Anti-BAFF/TNFSF13B Polyclonal Antibody is a Rabbit antibody targeting BAFF/TNFSF13B. Anti-BAFF/TNFSF13B Polyclonal Antibody can be used in WB,FCM,ELISA.

IgG

Human (predicted:Mouse,Rat,Pig,Cow,Rabbit)

1. Blank control (blue).: Jurkat cells (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-BAFF antibody (TMAB-00182), Dilution: 3 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
2. Measured by its binding ability in a indirect ELISA. Immobilized TNFSF13B Peptides at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-TNFSF13B Antibody, the EC50 is 797.5 ng/mL.
3. Sample:
Lane 1: Recombinant human TNFSF13B protein, N-His
Primary: Anti-TNFSF13B (TMAB-00182) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 52 kDa

WB,FCM,ELISA

WB: 1:500-2000; FCM: 3μg/Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: mouse TNFSF13B

Mouse

TNF,Death Ligands,Non-CD,Necrosis Factors