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Anti-PSGL-1/CD162 Antibody (6E322)
🥰Excellent
Catalog No. TMAY-02647
Anti-PSGL-1/CD162 Antibody (6E322) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (6E322) can be used in ELISA,IHC-P,FCM.
Anti-PSGL-1/CD162 Antibody (6E322)
Anti-PSGL-1/CD162 Antibody (6E322)
🥰Excellent
Catalog No. TMAY-02647
Anti-PSGL-1/CD162 Antibody (6E322) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (6E322) can be used in ELISA,IHC-P,FCM.
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Product Introduction
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PSGL-1/CD162 Antibody (6E322) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (6E322) can be used in ELISA,IHC-P,FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 6E322 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of Human SELPLG(CD162) expression on human whole blood granulocytes. Cells were stained with PE-conjugated anti-Human SELPLG(CD162). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes. 2. Immunochemical staining of human SELPLG in human tonsil with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). 3. Immunochemical staining of human SELPLG in human spleen with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). |
| Application | ELISA,IHC-P,FCM |
| Recommended Dose | ELISA: 1:1000-1:2000; IHC-P: 1:500-1:2000; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSGL-1 / CD162 / SELPLG (rh PSGL-1 / CD162 / SELPLG; TMPY-03531; Q14242-1; Met1-Gly295). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin. |
| Conjucates | Unconjugated |
| Others Formats | APC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human PSGL-1 / CD162 / SELPLG Protein (TMPY-03531) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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