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Anti-USP22 Antibody (6P785)

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Catalog No. TMAH-01238
Alias USP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22

Anti-USP22 Antibody (6P785) is an antibody targeting USP22. Anti-USP22 Antibody (6P785) can be used in ELISA, IF, FCM.

Anti-USP22 Antibody (6P785)

Anti-USP22 Antibody (6P785)

😃Good
Catalog No. TMAH-01238Alias USP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22
Anti-USP22 Antibody (6P785) is an antibody targeting USP22. Anti-USP22 Antibody (6P785) can be used in ELISA, IF, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IF, FCM

Reactivity: Human
AliasUSP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22
Ig Type
Rabbit IgG
Clone
6P785
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of MCF-7 with TMAH-01238 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 493-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
2. Overlay Peak curve showing Hela cells stained with TMAH-01238 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, IF, FCM
Recommended Dose
IF:1:50-1:200; FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundHistone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human USP22
Antigen Species
Human
Gene ID
23326
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling, Cell biology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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