Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human

Rabbit IgG

5H781

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
-All lanes: DNA Ligase I antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 102, 89, 99 kDa
-Observed band size: 140 kDa
2. IHC image of TMAH-00697 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00697 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

WB:1:500-1:5000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human DNA Ligase I

Human

3978

Epigenetics and Nuclear Signaling