Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IF, FC

Reactivity: Human

Rabbit IgG

2U963

Human

1. Western Blot
-Positive WB detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
-All lanes: DNAJA1 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 45, 38 kDa
-Observed band size: 45-55 kDa
2. Immunofluorescence staining of A549 cell with TMAH-00358 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 550-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Overlay Peak curve showing A549 cells stained with TMAH-00358 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IF, FC

WB:1:500-1:2000; IF:1:50-1:200; FC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human DNAJA1

Human

3301

Signal transduction