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Anti-GFP Antibody (1V694)
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Catalog No. TMAY-02488
Alias GFP Protein
Anti-GFP Antibody (1V694) is a Rabbit antibody targeting GFP. Anti-GFP Antibody (1V694) can be used in WB,ELISA,IP.
Anti-GFP Antibody (1V694)
Anti-GFP Antibody (1V694)
😃Good
Catalog No. TMAY-02488Alias GFP Protein
Anti-GFP Antibody (1V694) is a Rabbit antibody targeting GFP. Anti-GFP Antibody (1V694) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $93 | 7-10 days | |
| 100 μL | $186 | 7-10 days | |
| 500 μL | $469 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-GFP Antibody (1V694) is a Rabbit antibody targeting GFP. Anti-GFP Antibody (1V694) can be used in WB,ELISA,IP. |
| Alias | GFP Protein |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1V694 |
| Reactivity | Aequorea victoria |
| Verified Activity | 1. Anti-GFP Tag was immunoprecipitated using: -Lane A: GFP transfected E.coli cell lysate (0.5mg) -Lane B: GFP transfected 293 cell lysate (0.5mg) -Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Anti-GFP rabbit monoclonal antibody at 1:500 dilution. -Lane A: GFP transfected E.coli lysate (5ug) -Lane B: GFP transfected 293 Cell lysate (30ug) -Lane C: Non-transfected 293 Cells lysate (30ug) -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:27 kDa. -Observed band size:27 kDa. 3. Anti-GFP rabbit monoclonal antibody at 1:1000 dilution. -Sample: Recombinant Protein 10 ng -Lane 1: Aequorea victoria GFP Protein (His Tag) -Lane 2: EGFP / Enhanced Green Fluorescent Protein (His Tag) -Lane 3: Aequorea victoria Green fluorescent protein /enhanced GFP GFPSparkTM -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. 4. GFP was immunoprecipitated using: -Lane A:0.5 mg pCMV3-untagged Negative Control Vector -Lane B:0.5 mg pCMV3-C-GFPSpark Vector -Lane C:0.5 mg pCMV3-N-GFPSpark Vector -4 µL anti- GFP rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti- GFP rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:1000-1:5000; ELISA: 1:10000-1:20000; IP: 1-5 μl/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (TMPY-02451; AAB65663; Ser2-Lys238). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Green fluorescent protein (GFP tag) is a ~27 kDa protein consisting of 238 amino acids derived from jellyfish Aequorea Victoria. GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields. GFP tag antibodies are also often been used to study the expression of proteins and the interaction between proteins and proteins. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Aequorea victoria GFP protein (TMPY-02451) |
| Antigen Species | Aequorea victoria |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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