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Anti-Phospho-SMAD2 (Ser250) Antibody (7J329) is an antibody targeting Phospho-SMAD2 (Ser250). Anti-Phospho-SMAD2 (Ser250) Antibody (7J329) can be used in ELISA, WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB Reactivity: Human |
Alias | SMAD2 (p-Ser250), SMAD2 (p-S250), p-SMAD2 (Ser250), p-SMAD2 (S250), Phospho-SMAD2 (S250) |
Ig Type | Rabbit IgG |
Clone | 7J329 |
Reactivity | Human |
Verified Activity | Western Blot -Positive WB detected in Hela whole cell lysate,A549 whole cell lysate -All lanes Phospho-SMAD2 antibody at 1.07μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 58 KDa -Observed band size: 58 KDa |
Application | ELISA, WB |
Recommended Dose | WB:1:500-1:5000. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm. Nucleus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human Phospho-SMAD2 (S250) |
Antigen Species | Human |
Gene ID | 4087 |
Uniprot ID | |
Biology Area | Signal Transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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