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Anti-GFP Polyclonal Antibody is a Rabbit antibody targeting GFP. Anti-GFP Polyclonal Antibody can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $125 | 7-10 days | |
100 μL | $183 | 7-10 days | |
500 μL | $754 | 7-10 days |
Description | Anti-GFP Polyclonal Antibody is a Rabbit antibody targeting GFP. Anti-GFP Polyclonal Antibody can be used in WB. |
Synonyms | GFP Protein |
Ig Type | IgG |
Reactivity | Species independent |
Verified Activity | 1. Sample: GFP-Tagged fusion protein Overexpression E.coli Lysate at 4 μg Primary: Anti-GFP (TMAB-00765) at 1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 30 kDa 2. Sample: Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate at 4 μg Lane 2: Control (-) E.coli Lysate at 4 μg Primary: Anti-GFP (TMAB-00765) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa |
Application | |
Recommended Dose | WB: 1:20000-50000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Tissue Specificity | Photocytes. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration | 1 mg/mL |
Research Background | Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. |
Immunogen | KLH conjugated synthetic peptide: belt jellyfish GFP |
Gene Name | GFP |
Protein Name | Green fluorescent protein |
Biology Area | GFP |
Function | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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