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Anti-GFP Polyclonal Antibody

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Catalog No. TMAB-00765
Alias GFP Protein

Anti-GFP Polyclonal Antibody is a Rabbit antibody targeting GFP. Anti-GFP Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

Anti-GFP Polyclonal Antibody

Anti-GFP Polyclonal Antibody

😃Good
Catalog No. TMAB-00765Alias GFP Protein
Anti-GFP Polyclonal Antibody is a Rabbit antibody targeting GFP. Anti-GFP Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
100 μL$1067-10 days7-10 days
200 μL$1817-10 days7-10 days
500 μL$4267-10 days7-10 days
1 mL$8047-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-GFP Polyclonal Antibody is a Rabbit antibody targeting GFP. Anti-GFP Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
SynonymsGFP Protein
Ig Type
IgG
Reactivity
Species independent
Verified Activity
1. Sample:
GFP-Tagged fusion protein Overexpression E.coli Lysate at 4 μg
Primary:
Anti-GFP (TMAB-00765) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa
2. Sample:
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate at 4 μg
Lane 2: Control (-) E.coli Lysate at 4 μg
Primary: Anti-GFP (TMAB-00765) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
verifiedActivityverifiedActivity
Application
ELISA
FCM
ICC/IF
IF
IHC-Fr
IHC-P
WB
Recommended Dose
ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:20000-50000
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityPhotocytes.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundEnhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: belt jellyfish GFP
Gene Name
GFP
Protein Name
Green fluorescent protein
Biology Area
GFP
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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