B7-H2/ICOSLG Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00192 Copy Product Info

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. B7-H2/ICOSLG Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 29.5 kDa and the accession number is O75144.

B7-H2/ICOSLG Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00192

Copy Product Info

B7-H2/ICOSLG Protein, Human, Recombinant (His & Avi)

Pack Size	Price	USA Stock	Global Stock
5 μg	$35	7-10 days	7-10 days
10 μg	$52	7-10 days	7-10 days
20 μg	$81	7-10 days	7-10 days
50 μg	$153	7-10 days	7-10 days
100 μg	$255	7-10 days	7-10 days
200 μg	$459	7-10 days	7-10 days
500 μg	$1,020	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	1. Immobilized Human B7-H2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA. 2. Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay.
Description	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. B7-H2/ICOSLG Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 29.5 kDa and the accession number is O75144.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	O75144
Synonyms	LICOS,ICOSLG,ICOS-L,ICOSL,ICOS ligand,GL50,CD275,B7RP1B7-like protein Gl50,B7RP-1,B7RP1,B7-H2B7-related protein 1,B7-H2,B7H2
Construction	Asp19-Ser258
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	29.5 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.