B7-H2/ICOSLG Protein, Human, Recombinant (His & Avi)

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. B7-H2/ICOSLG Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 29.5 kDa and the accession number is O75144.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 255.00
500 μg	5 days	$ 1,020.00
1 mg	5 days	$ 1,700.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 255.00

500 μg

5 days

$ 1,020.00

1 mg

5 days

$ 1,700.00

Description

Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	O75144
Synonyms	B7RP1B7-like protein Gl50, B7-H2B7-related protein 1, ICOSLG, LICOS, B7RP-1, B7RP1, ICOSL, CD275, ICOS-L, GL50, ICOS ligand, B7H2, B7-H2
Construction	Asp19-Ser258
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	29.5 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.