Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC, IF

Reactivity: Human

Rabbit IgG

6Y581

Human

1. Western Blot
-Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate, Raji whole cell lysate, Colo320 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate
-All lanes: FTO antibody at 0.7μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 59, 15, 7, 13 KDa
-Observed band size: 59 KDa
2. IHC image of TMAH-00463 diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. IHC image of TMAH-00463 diluted at 1:70 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4. Immunofluorescence staining of Hela cells with TMAH-00463 at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ELISA, WB, IHC, IF

WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human FTO

Human

79068

Neuroscience