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Anti-ILK Antibody (3J462)
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Catalog No. TMAH-00631
Anti-ILK Antibody (3J462) is an antibody targeting ILK. Anti-ILK Antibody (3J462) can be used in ELISA, WB, IF, IP.
Anti-ILK Antibody (3J462)
Anti-ILK Antibody (3J462)
😃Good
Catalog No. TMAH-00631
Anti-ILK Antibody (3J462) is an antibody targeting ILK. Anti-ILK Antibody (3J462) can be used in ELISA, WB, IF, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | 198 € | 7-10 days | |
| 100 μL | 329 € | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, IF, IP Reactivity: Human, Mouse |
| Ig Type | Rabbit IgG |
| Clone | 3J462 |
| Reactivity | Human, Mouse |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue -All lanes: ILK antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 52, 45, 37 kDa -Observed band size: 51 kDa 2. Immunofluorescence staining of MCF7 Cells with TMAH-00631 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). 3. Immunoprecipitating ILK in Hela whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00631 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00631(2µg)+ Hela whole cell lysate(500µg) -Lane 3: Hela whole cell lysate (10µg) |
| Application | ELISA, WB, IF, IP |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cytoplasm, myofibril, sarcomere. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Regulates cell motility by forming a complex with PARVB. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human ILK |
| Antigen Species | Human |
| Gene ID | 3611 |
| Uniprot ID | |
| Biology Area | Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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