Anti-ILK Antibody (3J462) is an antibody targeting ILK. Anti-ILK Antibody (3J462) can be used in ELISA,IF,IP,WB.

Rabbit IgG

3J462

Human, Mouse

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
-All lanes: ILK antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 52, 45, 37 kDa
-Observed band size: 51 kDa
2. Immunofluorescence staining of MCF7 Cells with TMAH-00631 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
3. Immunoprecipitating ILK in Hela whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00631 in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00631(2µg)+ Hela whole cell lysate(500µg)
-Lane 3: Hela whole cell lysate (10µg)

WB:1:500-1:5000; IF:1:20-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human ILK

Human

ILK

Signal transduction