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Anti-SAE1 Antibody (8M685)
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Catalog No. TMAY-02674
Alias UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1
Anti-SAE1 Antibody (8M685) is a Mouse antibody targeting SAE1. Anti-SAE1 Antibody (8M685) can be used in ELISA,ICC/IF.
Anti-SAE1 Antibody (8M685)
Anti-SAE1 Antibody (8M685)
😃Good
Catalog No. TMAY-02674Alias UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1
Anti-SAE1 Antibody (8M685) is a Mouse antibody targeting SAE1. Anti-SAE1 Antibody (8M685) can be used in ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SAE1 Antibody (8M685) is a Mouse antibody targeting SAE1. Anti-SAE1 Antibody (8M685) can be used in ELISA,ICC/IF. |
| Alias | UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1 |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 8M685 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of SAE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Y SAE1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus. |
| Application | ELISA,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SAE1 (rh SAE1; TMPY-03062; Q9UBE0; Met1-Lys346). The IgG fraction of the cell culture supernatant was purified by Protein A affinity. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human SAE1 protein (TMPY-03062) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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