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TOPBioactivityRelated Conjugates and FormulationsAntigen DetailsChemical infoStorage&SolubilityCalculator

Anti-ITGAL Antibody (6D918)

😃Good
Catalog No. TMAH-00645
Alias CD11A

Anti-ITGAL Antibody (6D918) is an antibody targeting ITGAL. Anti-ITGAL Antibody (6D918) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.

Anti-ITGAL Antibody (6D918)

Anti-ITGAL Antibody (6D918)

😃Good
Catalog No. TMAH-00645Alias CD11A
Anti-ITGAL Antibody (6D918) is an antibody targeting ITGAL. Anti-ITGAL Antibody (6D918) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Instruction Manual of Detection Antibody
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF

Reactivity: Human
AliasCD11A
Ig Type
Rabbit IgG
Clone
6D918
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: THP-1 whole cell lysate
-All lanes: ITGAL antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 129, 135, 120 kDa
-Observed band size: 130 kDa
2. Immunofluorescence staining of THP-1 cell with TMAH-00645 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application
ELISA, WB, IF
Recommended Dose
WB:1:500-1:2000; IF:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundIntegrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R. Integin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human ITGAL
Antigen Species
Human
Gene ID
3683
Uniprot ID
Biology Area
Immunology, Signal transduction, Stem cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

LFA-1ALFA1ALeukocyte function-associated molecule 1 α chainLeukocyte function-associated molecule 1 alpha chainLeukocyte function-associated molecule 1 a chainLeukocyte adhesion glycoprotein LFA-1 α chain (LFA-1A)Leukocyte adhesion glycoprotein LFA-1 alpha chain (LFA-1A)Leukocyte adhesion glycoprotein LFA-1 a chain (LFA-1A)ITGALIntegrinα-LIntegrinαLIntegrinaLIntegrin α-LIntegrin αLIntegrin alpha-LIntegrin alphaLIntegrin a-LIntegrin aLCD11aCD11 antigen-like family member A
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