Antibody Type: Recombinant Monoclonal

Application: ELISA, IHC, FCM

Reactivity: Human

hIgG1

9H202

Human

1. IHC image of TMAH-00669 diluted at 1：100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
2. Overlay Peak curve showing JK cells stained with TMAH-00669 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, IHC, FCM

IHC:1:50-1:200; FCM:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human KIT Protein

Human

3815

Neuroscience, Cancer, Developmental biology, Tags & Cell Markers, Immunology, Signal transduction, Stem cells