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Anti-B7-H3 Antibody (8G959)

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Catalog No. TMAH-00187
Alias UNQ309, PSEC0249, PRO352, CD276, B7-H3, B7 homolog 3,  4Ig-B7-H3

Anti-B7-H3 Antibody (8G959) is an antibody targeting B7-H3. Anti-B7-H3 Antibody (8G959) can be used in ELISA.

Anti-B7-H3 Antibody (8G959)

Anti-B7-H3 Antibody (8G959)

😃Good
Catalog No. TMAH-00187Alias UNQ309, PSEC0249, PRO352, CD276, B7-H3, B7 homolog 3,  4Ig-B7-H3
Anti-B7-H3 Antibody (8G959) is an antibody targeting B7-H3. Anti-B7-H3 Antibody (8G959) can be used in ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Monoclonal

Application: ELISA

Reactivity: Human, Cynomolgus
AliasUNQ309, PSEC0249, PRO352, CD276, B7-H3, B7 homolog 3,  4Ig-B7-H3
Ig Type
hIgG1
Clone
8G959
Reactivity
Human, Cynomolgus
Verified Activity
1. The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276 at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC50 is 4.299-5.373 ng/mL.
2. The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276 at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC50 is 47.12-54.16 ng/mL.
3. Overlay Peak curve showing MCF7 cells stained with TMAH-00187MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA
Recommended Dose
ELISA:1:5000-1:50000.
Antibody Type
Monoclonal
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Research BackgroundEarlier studies found that B7H3 (also known as B7H3) promotes the activation of T cells. Chapoval et al. confirmed that in the presence of anti-CD3 antibodies, B7H3 can promote the proliferation of CD4 and CD8+ T cells and selectively promote the secretion of IFN-γ. And B7H3 transfection into tumor cells can enhance the killing ability of CTL. Further research found that only TLT-2 transgenic cells could bind to mouse B7H3 with high affinity, and TLT-2 was determined to be the receptor molecule of B7H3. Moreover, Hashiguchi et al. confirmed that the B7H3-TLT-2 pathway enhanced T cell activation. However, Leitner et al. did not find the specific binding of B7H3 to TLT-2 by flow cytometry, therefore, the exact receptor molecule of B7H3 is still unclear. On the other hand, studies have found that B7H3 can also suppress T-cell immune responses. Some studies have shown that B7H3 can inhibit human and mouse T cells by activating or inhibiting NFTA (nuclear factor for activated T cells), NF-KB (nuclear factor kB) and AP-1 (activator protein-1) pathways. activation. In addition, results have demonstrated that B7H3 may inhibit T cell immune responses by inhibiting the activity of Thl. The study of Leiner et al. also found that B7H3 can down-regulate the secretion of IL-2 in T cells to inhibit the activity of T cells.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CD276 Protein
Antigen Species
Human
Gene ID
80381
Uniprot ID
Biology Area
Cancer, Immunology, Stem cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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