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Claudin-4 Protein-Nanodisc, Human, Recombinant (Avi & His), Biotinylated

(Synonyms: WBSCR8, hCPE-R, CPETR1, CPETR, CPE-R, CPER) Copy Product Info

Synonyms: WBSCR8, hCPE-R, CPETR1, CPETR, CPE-R, CPER

Catalog No. TMPY-07141 Copy Product Info
Claudin-4 Protein-Nanodisc, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 Cells. The accession number is O14493.
Claudin-4 Protein-Nanodisc, Human, Recombinant (Avi & His), Biotinylated
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Pack SizePriceUSA StockGlobal StockQuantity
5 μg$8157-10 days7-10 days
10 μg$1,3707-10 days7-10 days
20 μg$2,3207-10 days7-10 days
50 μg$4,7607-10 days7-10 days
100 μg$9,4607-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Claudin-4 Protein-Nanodisc, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 Cells. The accession number is O14493.
Species
Human
Expression System
HEK293 Cells
TagC-His, N-Avi
Accession NumberO14493
ConstructionA DNA sequence encoding the Human CLDN4 (O14493) (Ala2-Val209) was expressed with an AVI tag at the N-terminus and a polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Protein Purity
≥ 80% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationSupplied as sterile 50 mM HEPES, 150 mM NaCl, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability & StorageSamples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
ShippingProteins are shipped with blue ice.
SynonymsWBSCR8, hCPE-R, CPETR1, CPETR, CPE-R, CPER
Research Background
Chemical Properties
Molecular Weight25.52 kDa (predicted); 23.4 kDa (reducing contition)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Claudin-4 Protein-Nanodisc, Human, Recombinant (Avi & His), Biotinylated chemical structure | Claudin-4 Protein-Nanodisc, Human, Recombinant (Avi & His), Biotinylated molecular weight