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Anti-GUCY2C Antibody (4L52) is an antibody targeting GUCY2C. Anti-GUCY2C Antibody (4L52) can be used in ELISA, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, FCM Reactivity: Human |
Alias | STAR, STA receptor, MUCIL, GUCY2C, GUC2CEC 4.6.1.2, GUC2C, Guanylyl cyclase C, GC-C, GCC, EC 4.6.1, DIAR6 |
Ig Type | Human IgG1 |
Clone | 4L52 |
Reactivity | Human |
Verified Activity | 1. The Binding Activity of Human GUCY2C with Anti-GUCY2C recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody, the EC50 is 5.630-9.996 ng/mL. 2. T84 cells were stained with Human IgG (green line) and anti-GUCY2C antibody (red line) (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry. 3. Untransfected CT26 cells surface (green line) and transfected Human GUCY2C CT26 stable cells surface (red line) were stained with anti-GUCY2C antibody (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry. |
Application | ELISA, FCM |
Recommended Dose | FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human GUCY2C Protein |
Antigen Species | Human |
Gene ID | 2984 |
Uniprot ID | |
Biology Area | Immunology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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