1. Loaded Human 4-1BB-Fc-His on Protein A Biosensor, can bind Human 4-1BBL-His with an affinity constant of 1.55 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Human 4-1BBL-His at 10μg/ml (100 μl/well) can bind Human 4-1BB-Fc . The ED50 of Human 4-1BB-Fc  is 16.8 ng/ml. (Regularly tested)

4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16-20 KDa and the accession number is P41273.

Human

E. coli

Tumor necrosis factor ligand superfamily member 9,TNFSF9,4-1BBL,4-1BB ligand

Arg71-Glu254

Arg71-Glu254

Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.