4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His)

Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.

Pack Size	Availability	Price/USD
10 μg	In stock	$ 83.00
50 μg	5 days	$ 250.00
500 μg	5 days	$ 1,220.00
1 mg	5 days	$ 2,730.00

Pack Size

Availability

Price/USD

Quantity

10 μg

In stock

$ 83.00

50 μg

5 days

$ 250.00

500 μg

5 days

$ 1,220.00

1 mg

5 days

$ 2,730.00

Biological Information

1. Loaded Human 4-1BB-Fc-His on Protein A Biosensor, can bind Human 4-1BBL-His with an affinity constant of 1.55 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Human 4-1BBL-His at 10μg/ml (100 μl/well) can bind Human 4-1BB-Fc . The ED50 of Human 4-1BB-Fc is 16.8 ng/ml. (Regularly tested)

Description

Species	Human
Expression System	E. coli
Tag	C-6His
Accession Number	P41273
Synonyms	4-1BBL, Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, TNFSF9
Amino Acid	Arg71-Glu254
Construction	Recombinant Human 4-1BB Ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Molecular Weight	16-20 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.

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4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His)

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