SEMA4D Protein, Mouse, Recombinant (His)

SEMA4D Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 80.2 kDa and the accession number is O09126.

Pack Size	Availability	Price/USD	Quantity
100 μg	5 days	$ 600.00
1 mg	5 days	$ 3,910.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 600.00

1 mg

5 days

$ 3,910.00

Description

SEMA4D Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 80.2 kDa and the accession number is O09126.

Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	O09126
Synonyms	Semacl2, coll-4, CD100, semaphorin 4D, Semaj, Semcl2
Construction	The Mouse SEMA4D (NP_038688.2) extracellular domain (Met 1-Arg 733) was expressed, with a C-terminal polyhistidine tag.
Protein Purity	> 96 % as determined by SDS-PAGE
Molecular Weight	80.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.

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SEMA4D Protein, Mouse, Recombinant (His)

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