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Anti-HNMT Antibody (7C164)

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Catalog No. TMAY-02476
Alias HNMT-S2, HNMT-S1, HMT, histamine N-methyltransferase

Anti-HNMT Antibody (7C164) is a Rabbit antibody targeting HNMT. Anti-HNMT Antibody (7C164) can be used in WB,ELISA,IP.

Anti-HNMT Antibody (7C164)

Anti-HNMT Antibody (7C164)

😃Good
Catalog No. TMAY-02476Alias HNMT-S2, HNMT-S1, HMT, histamine N-methyltransferase
Anti-HNMT Antibody (7C164) is a Rabbit antibody targeting HNMT. Anti-HNMT Antibody (7C164) can be used in WB,ELISA,IP.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Anti-HNMT Antibody (7C164) is a Rabbit antibody targeting HNMT. Anti-HNMT Antibody (7C164) can be used in WB,ELISA,IP.
AliasHNMT-S2, HNMT-S1, HMT, histamine N-methyltransferase
Ig Type
Monoclonal Rabbit IgG
Clone
7C164
Reactivity
Human
Verified Activity
1. Anti-HNMT rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:33 kDa.

-Observed band size:33 kDa.

2. HNMT was immunoprecipitated using:

-Lane A:0.5 mg HepG2 Whole Cell Lysate.

-Lane B:0.5 mg Hela Whole Cell Lysate.

-2 µL anti-HNMT rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-HNMT rabbit monoclonal antibody, at 1:200 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 33 kDa.

-Observed band size: 33 kDa
Application
WB,ELISA,IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human HNMT (rh HNMT; TMPY-02340; AAH20677.1; Met1-Ala292).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundHNMT (Histamine N-methyltransferase) is a Protein Coding gene. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT, the major enzyme for the metabolism of histamine in the rat brain, is potently inhibited by 9-amino-1,2,3,4-tetrahydroacridine (tacrine). Methylation is an important pathway in the biotransformation of many drugs, neurotransmitters, and xenobiotic compounds. Histamine N-methyltransferase (HNMT) catalyzes the N tau-methylation of histamine and structurally related compounds. Histamine N-methyltransferase (HNMT) is believed to be the sole pathway for termination of the neurotransmitter action of histamine in the mammalian brain. That highlights the importance of the inclusion of HNMT for genetic testing of individuals presenting with intellectual disability.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human HNMT protein (TMPY-02340)
Antigen Species
Human
Biology Area
Neurotransmitter Associated Enzymes
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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