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Anti-HNMT Antibody (7C164) is a Rabbit antibody targeting HNMT. Anti-HNMT Antibody (7C164) can be used in WB,ELISA,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $268 | 7-10 days |
Description | Anti-HNMT Antibody (7C164) is a Rabbit antibody targeting HNMT. Anti-HNMT Antibody (7C164) can be used in WB,ELISA,IP. |
Alias | HNMT-S2, HNMT-S1, HMT, histamine N-methyltransferase |
Ig Type | Monoclonal Rabbit IgG |
Clone | 7C164 |
Reactivity | Human |
Verified Activity | 1. Anti-HNMT rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:33 kDa. 2. HNMT was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -2 µL anti-HNMT rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HNMT rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size: 33 kDa |
Application | WB,ELISA,IP |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human HNMT (rh HNMT; TMPY-02340; AAH20677.1; Met1-Ala292). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | HNMT (Histamine N-methyltransferase) is a Protein Coding gene. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT, the major enzyme for the metabolism of histamine in the rat brain, is potently inhibited by 9-amino-1,2,3,4-tetrahydroacridine (tacrine). Methylation is an important pathway in the biotransformation of many drugs, neurotransmitters, and xenobiotic compounds. Histamine N-methyltransferase (HNMT) catalyzes the N tau-methylation of histamine and structurally related compounds. Histamine N-methyltransferase (HNMT) is believed to be the sole pathway for termination of the neurotransmitter action of histamine in the mammalian brain. That highlights the importance of the inclusion of HNMT for genetic testing of individuals presenting with intellectual disability. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human HNMT protein (TMPY-02340) |
Antigen Species | Human |
Biology Area | Neurotransmitter Associated Enzymes |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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