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Anti-Caspase-3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00285
Anti-Caspase-3 Polyclonal Antibody is a Rabbit antibody targeting Caspase-3. Anti-Caspase-3 Polyclonal Antibody can be used in WB,ELISA.
Anti-Caspase-3 Polyclonal Antibody
Anti-Caspase-3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00285
Anti-Caspase-3 Polyclonal Antibody is a Rabbit antibody targeting Caspase-3. Anti-Caspase-3 Polyclonal Antibody can be used in WB,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,ELISA Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Raji (Human) Cell Lysate at 30 μg Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 3: Lung (Mouse) Lysate at 40 μg Lane 4: Lung (Rat) Lysate at 40 μg Primary: Anti-Caspase-3 (TMAB-00285) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 37 kDa 2. Sample: Lane 1: Spleen (Mouse) Lysate at 40 μg Lane 2: Lung (Mouse) Lysate at 40 μg Lane 3: LympHnode (Mouse) Lysate at 40 μg Lane 4: Cerebrum (Mouse) Lysate at 40 μg Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 6: Spleen (Rat) Lysate at 40 μg Lane 7: Lung (Rat) Lysate at 40 μg Lane 8: LympHnode (Rat) Lysate at 40 μg Lane 9: Cerebrum (Rat) Lysate at 40 μg Lane 10: Hela (Human) Cell Lysate at 30 μg Primary: Anti-Caspase-3 (TMAB-00285) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 37 kDa |
| Application | WB,ELISA |
| Recommended Dose | WB: 1:500-2000; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. |
| Immunogen | KLH conjugated synthetic peptide: human caspase-3 p17 subunit |
| Antigen Species | Human |
| Gene ID | 836 |
| Uniprot ID | |
| Biology Area | Caspases,Metabolism,Caspases,Caspases,Other proteases,Apoptosis |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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