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Anti-GLS1 Polyclonal Antibody

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Catalog No. TMAB-00779
Alias L-glutamine amidohydrolase, KIAA0838, K-glutaminase, KGA, Glutaminase kidney isoform, mitochondrial, GLSK, GLS, GDPAG, GAM, GAC, EIEE71, EC:3.5.1.2, DEE71, CASGID, AAD20

Anti-GLS1 Polyclonal Antibody is a Rabbit antibody targeting GLS1. Anti-GLS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-GLS1 Polyclonal Antibody

Anti-GLS1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00779Alias L-glutamine amidohydrolase, KIAA0838, K-glutaminase, KGA, Glutaminase kidney isoform, mitochondrial, GLSK, GLS, GDPAG, GAM, GAC, EIEE71, EC:3.5.1.2, DEE71, CASGID, AAD20
Anti-GLS1 Polyclonal Antibody is a Rabbit antibody targeting GLS1. Anti-GLS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
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Product Introduction

Bioactivity
Description
Anti-GLS1 Polyclonal Antibody is a Rabbit antibody targeting GLS1. Anti-GLS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
AliasL-glutamine amidohydrolase, KIAA0838, K-glutaminase, KGA, Glutaminase kidney isoform, mitochondrial, GLSK, GLS, GDPAG, GAM, GAC, EIEE71, EC:3.5.1.2, DEE71, CASGID, AAD20
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Cow)
Verified Activity
1. Sample:
Lane 1: Human U251 cell lysates
Lane 2: Human HeLa cell lysates
Primary: Anti-GLS1 (TMAB-00779) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 75 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (TMAB-00779) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (TMAB-00779) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GLS1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Amino acid metabolism,Glutamate,Amino Acids
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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