TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (His & Avi), Biotinylated

Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 814.00
500 μg	5 days	$ 3,250.00
1 mg	5 days	$ 5,440.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 814.00

500 μg

5 days

$ 3,250.00

1 mg

5 days

$ 5,440.00

Description

Species	Human
Expression System	HEK293
Tag	C-His-Avi
Accession Number	Q8TDQ0-1
Synonyms	TIM 3, HAVCR2, TIMD-3, TIM3, KIM3, CD366, TIM-3, FLJ14428
Construction	Recombinant Biotinylated Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser22-Arg200.[Accession \|Q8TDQ0-1]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.