**HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Human, MHC (His & Avi), Biotinylated**

Catalog No. TMPK-01547

Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.

**HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Human, MHC (His & Avi), Biotinylated**

Catalog No. TMPK-01547

Pack Size	Price	Availability	Quantity
100 μg	$810	7-10 days
1 mg	$5,400	7-10 days

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Product Information

Biological Activity	Immobilized Anti-HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag with the EC50 of 0.19μg/ml determined by ELISA.
Description	Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV
Synonyms	MHC I,member 4,MAGE-X2,MAGE-A4,MAGE4B,MAGE4A,HLA0201,CT1.4
Construction	Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.