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Anti-ALDH4A1 Antibody (6Q90)
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Catalog No. TMAY-02423
Anti-ALDH4A1 Antibody (6Q90) is a Rabbit antibody targeting ALDH4A1. Anti-ALDH4A1 Antibody (6Q90) can be used in WB,ELISA,IP.
Anti-ALDH4A1 Antibody (6Q90)
Anti-ALDH4A1 Antibody (6Q90)
😃Good
Catalog No. TMAY-02423
Anti-ALDH4A1 Antibody (6Q90) is a Rabbit antibody targeting ALDH4A1. Anti-ALDH4A1 Antibody (6Q90) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-ALDH4A1 Antibody (6Q90) is a Rabbit antibody targeting ALDH4A1. Anti-ALDH4A1 Antibody (6Q90) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6Q90 |
| Reactivity | Human |
| Verified Activity | 1. Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:62 kDa. -Observed band size:62 kDa(We are unsure as to the identity of these extra bands.) 2. ALDH4A1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate. -2 µL anti-ALDH4A1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ALDH4A1 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 62 kDa. -Observed band size: 62 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (rh ALDH4A1; TMPY-02799; AAH07581.1; Lys25-Gln563). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human ALDH4A1 Protein (TMPY-02799) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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