Nectin-2 Protein, Human, Recombinant (His)

Catalog No. TMPY-00747

Nectin-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.2 kDa and the accession number is Q92692-2.

Nectin-2 Protein, Human, Recombinant (His)

Catalog No. TMPY-00747

Nectin-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.2 kDa and the accession number is Q92692-2.

Pack Size	Price	Availability
100 μg	$376	In Stock
200 μg	$665	7-10 days
500 μg	$1,410	7-10 days

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Product Information

Biological Activity	1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD112 at 20 μg/ml (100 μl/well) can bind biotinylated DNAM1 with a linear range of 0.078-2.5 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of Anti-CD112 Antibody bound to Nectin-2 Protein, Human, Recombinant (His Tag) was 34.4 nM.
Description	Nectin-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.2 kDa and the accession number is Q92692-2.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q92692-2
Synonyms	PVRR2,PRR2,poliovirus receptor-related 2 (herpesvirus entry mediator B),Nectin-2,HVEB,CD112
Construction	A DNA sequence encoding the extracellular domain (Met 1-Leu 360) of human CD112 (NP_002847.1) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gln 32
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Molecular Weight	36.2 kDa (predicted); 48 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.