HER2/ERBB2 Protein, Human, Recombinant , Biotinylated

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD
20 μg	In stock	$ 197.00
100 μg	5 days	$ 468.00
200 μg	5 days	$ 797.00
500 μg	5 days	$ 1,610.00

Pack Size

Availability

Price/USD

Quantity

20 μg

In stock

$ 197.00

100 μg

5 days

$ 468.00

200 μg

5 days

$ 797.00

500 μg

5 days

$ 1,610.00

Biological Information

1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD), Biotinylated at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 10-50 ng/mL. 2. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated bound to Pertuzumab was 0.1 nM.

Description

Species	Human
Expression System	HEK293
Tag	Tag Free
Accession Number	P04626-1
Synonyms	CD340, ENVW, ENV, HER2, HERV7Q, HERVWENV, HERV-7q, HER-2, TKR1, EGFR2, HERVW, NGL, NEU, erb-b2 receptor tyrosine kinase 2, MLN 19, HER-2/neu, HERV-W-ENV, ERVWE1, MLN19
Construction	A DNA sequence encoding the human ERBB2 (NP_004439.2) (Met1-Thr652) was expressed with five amino acids (DDDDK) at the C-terminus. The purified protein was biotinylated in vitro.
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	Approxiamtely 70 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy