Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin is a Biotin-conjugated Goat antibody targeting IgG H&L. Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin can be used in WB,IHC-P,IHC-F,ELISA,ICC/IF,IF,FCM.

IgG

Mouse

1. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLP-1) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:1000) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:2000) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:2000) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:1000) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:500) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:2000) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:500) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:500) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLP-1) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:1000) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010B 1:500) instructionsand DAB staining.

WB: 1:2000-20000; IHC-P: 1:200-1000; IHC-F: 1:200-1000; ELISA: 1:2000-20000; ICC/IF: 1:100-1000; IF: 1:100-1000; FCM: 1:100-1000

Polyclonal

Native Mouse IgG