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Anti-TPSB2 Antibody (4C309)

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Catalog No. TMAH-01210

Anti-TPSB2 Antibody (4C309) is an antibody targeting TPSB2. Anti-TPSB2 Antibody (4C309) can be used in ELISA, WB, FCM.

Anti-TPSB2 Antibody (4C309)

Anti-TPSB2 Antibody (4C309)

😃Good
Catalog No. TMAH-01210
Anti-TPSB2 Antibody (4C309) is an antibody targeting TPSB2. Anti-TPSB2 Antibody (4C309) can be used in ELISA, WB, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, FCM

Reactivity: Human, Mouse
Ig Type
Rabbit IgG
Clone
4C309
Reactivity
Human, Mouse
Verified Activity
1. Western Blot
-Positive WB detected in: Mouse Kidney tissue lysate,
-All lanes: TPSB2 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 30 kDa
-Observed band size: 30 kDa
2. Overlay Peak curve showing K562 cells stained with TMAH-01210 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, WB, FCM
Recommended Dose
WB:1:500-1:2000; FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationSecreted. Note=Released from the secretory granules upon mast cell activation.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundTryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human TPSB2
Antigen Species
Human
Gene ID
64499
Uniprot ID
Biology Area
Others
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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