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Anti-14-3-3 eta/YWHAH Antibody (8D763)

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Catalog No. TMAY-01252
Alias YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta

Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in WB,ELISA,IP.

Anti-14-3-3 eta/YWHAH Antibody (8D763)

Anti-14-3-3 eta/YWHAH Antibody (8D763)

😃Good
Catalog No. TMAY-01252Alias YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in WB,ELISA,IP.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in WB,ELISA,IP.
AliasYWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Ig Type
Monoclonal Mouse IgG1
Clone
8D763
Reactivity
Human
Verified Activity
1. Anti-YWHAH mouse monoclonal antibody at 1:500 dilution.

-Lane A: U937 Whole Cell Lysate.

-Lane B: 293T Whole Cell Lysate.

-Lane C: Jurkat Whole Cell Lysate.

-Lane D: K562 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:28 kDa.

-Observed band size:30 kDa.

2. YWHAH was immunoprecipitated using:

-Lane A:0.5 mg Jurkat Whole Cell Lysate.

-Lane B:0.5 mg k562 Whole Cell Lysate.

-2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-YWHAH mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 28 kDa.

-Observed band size: 28 kDa
Application
WB,ELISA,IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; TMPY-02377; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundYWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region that has been implicated by linkage studies in both BP and schizophrenia.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human 14-3-3 eta / YWHAH protein (TMPY-02377)
Antigen Species
Human
Biology Area
phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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