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Anti-14-3-3 eta/YWHAH Antibody (8D763) (Synonyms: YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase ac...)

Catalog No. TMAY-01252 Copy Product Info
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Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in ELISA,IP,WB.

Anti-14-3-3 eta/YWHAH Antibody (8D763)

Copy Product Info
🥰Excellent
Catalog No. TMAY-01252
Synonyms YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta

Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in ELISA,IP,WB.

Anti-14-3-3 eta/YWHAH Antibody
(8D763)
Pack SizePriceUSA StockGlobal StockQuantity
100 μL$1997-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in ELISA,IP,WB.
SynonymsYWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Ig Type
Mouse IgG1
Clone
8D763
Reactivity
Human
Specificity
Human 14-3-3 eta/YWHAH
No cross-reactivity in ELISA with
E.coli cell lysate
Verified Activity
1. Anti-YWHAH mouse monoclonal antibody at 1:500 dilution.
-Lane A: U937 Whole Cell Lysate.
-Lane B: 293T Whole Cell Lysate.
-Lane C: Jurkat Whole Cell Lysate.
-Lane D: K562 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:28 kDa.
-Observed band size:30 kDa.
2. YWHAH was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg k562 Whole Cell Lysate.
-2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-YWHAH mouse monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 28 kDa.
-Observed band size: 28 kDa
Application
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; TMPY-02377; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundYWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region that has been implicated by linkage studies in both BP and schizophrenia.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human 14-3-3 eta / YWHAH protein (TMPY-02377)
Antigen Species
Human
Biology Area
phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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