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Anti-14-3-3 eta/YWHAH Antibody (8D763)
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Catalog No. TMAY-01252
Alias YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in WB,ELISA,IP.
Anti-14-3-3 eta/YWHAH Antibody (8D763)
Anti-14-3-3 eta/YWHAH Antibody (8D763)
😃Good
Catalog No. TMAY-01252Alias YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Anti-14-3-3 eta/YWHAH Antibody (8D763) is a Mouse antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (8D763) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA, IP Reactivity: Human |
| Alias | YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta |
| Ig Type | Mouse IgG1 |
| Clone | 8D763 |
| Reactivity | Human |
| Verified Activity | 1. Anti-YWHAH mouse monoclonal antibody at 1:500 dilution. -Lane A: U937 Whole Cell Lysate. -Lane B: 293T Whole Cell Lysate. -Lane C: Jurkat Whole Cell Lysate. -Lane D: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:28 kDa. -Observed band size:30 kDa. 2. YWHAH was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg k562 Whole Cell Lysate. -2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-YWHAH mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 28 kDa. -Observed band size: 28 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; TMPY-02377; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | YWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region that has been implicated by linkage studies in both BP and schizophrenia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human 14-3-3 eta / YWHAH protein (TMPY-02377) |
| Antigen Species | Human |
| Biology Area | phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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