Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF, FCM

Reactivity: Human

Rabbit IgG

9F685

Human

1. Western Blot
-Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate
-All lanes: ACLY antibody at 1:1500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 121, 120, 92 kDa
-Observed band size: 120 kDa
2. Immunofluorescence staining of HepG2 Cells with TMAH-00015 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
3. Overlay histogram showing Hela cells stained with TMAH-00015 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IF, FCM

WB:1:500-1:5000; IF:1:20-1:200; FCM:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human ATP citrate lyase

Human

47

Cancer, Metabolism, Signal transduction